| Gene | Vsx2 |
| Chromosome | 12 |
| Deletion Start | 84,569,946 |
| Deletion End | 84,593,508 |
| Deletion Size | 23,563 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,570,803 | 84,570,821 | TTCCTCCCGCTACGAACTG |
| TUP | 84,570,836 | 84,570,859 | CCATGGTACCCAGAGGGCAGCCAT |
| TUR | 84,570,865 | 84,570,883 | CCGGTGCCGCTTAGGTTAG |
| TDF | 84,592,683 | 84,592,702 | TGTCCCTTTGGTCACTTCTC |
| TDP | 84,592,704 | 84,592,729 | AGCTTTGCCCAAGAACTTCTTTGCCC |
| TDR | 84,592,754 | 84,592,772 | GCCTGGAGGGCAACTGATG |
PCR Primers
| Primer | Sequence |
| SU | ACCTGGCTGTTTCACCTCTG |
| SD | GCGAGAAGGAACAGGTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14525A-D4 | VGB6 | C57BL/6NTac | |
| 14525A-F2 | VGB6 | C57BL/6NTac | |
| 14525A-G10 | VGB6 | C57BL/6NTac | |
| 14525A-H9 | VGB6 | C57BL/6NTac | |
| 14525A-B12 | VGB6 | C57BL/6NTac | |
| 14525A-C10 | VGB6 | C57BL/6NTac | |
