| Gene | Vrtn |
| Chromosome | 12 |
| Deletion Start | 84,648,481 |
| Deletion End | 84,650,728 |
| Deletion Size | 2,248 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,648,531 | 84,648,552 | AGTGGAGTCCGAGGGCTTAGAG |
| TUP | 84,648,555 | 84,648,576 | TCTCATCGGTGCTGCCCTGGAG |
| TUR | 84,648,589 | 84,648,612 | GATGGGAAGGGTGAAGGAAGATAG |
| TDF | 84,650,511 | 84,650,531 | GCTGGTGATGGACATGCTTAC |
| TDP | 84,650,536 | 84,650,557 | ACGAGGTTCAAGGCCCAGGCCA |
| TDR | 84,650,601 | 84,650,621 | AGCGCCTGGAACTCCTTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GTCTATGGGCTTTTTGTCTTG |
| SD | TGTGATACTTTCCTTGTGGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 111 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14528A-A3 | VGB6 | C57BL/6NTac | |
| 14528A-A8 | VGB6 | C57BL/6NTac | |
| 14528A-E10 | VGB6 | C57BL/6NTac | |
| 14528A-F4 | VGB6 | C57BL/6NTac | |
| 14528A-F5 | VGB6 | C57BL/6NTac | |
| 14528A-H2 | VGB6 | C57BL/6NTac | |
