| Gene | BC030500 |
| Chromosome | 8 |
| Deletion Start | 58,912,512 |
| Deletion End | 58,913,206 |
| Deletion Size | 695 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 58,912,633 | 58,912,651 | GCTGGCGATGATACTGGTG |
| TUP | 58,912,652 | 58,912,672 | TCTACCACGTGCGGGAGCCTA |
| TUR | 58,912,733 | 58,912,757 | TCTGGGTTTGTTTATGTAGCATCTG |
| TDF | 58,913,076 | 58,913,097 | CGTAGCGTGGAAGGAGAAAGAG |
| TDP | 58,913,099 | 58,913,118 | ATTCCCACGCCGCGTGGACA |
| TDR | 58,913,121 | 58,913,139 | GGAAGCTGCGGCTAACAAC |
PCR Primers
| Primer | Sequence |
| SU | AAGGAGGCTACAGATGTCAG |
| SD | TACTTAAGTTCACACCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 125 bp | + | + | - |
| TDF | TDR | 64 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14536A-B3 | VGB6 | C57BL/6NTac | |
| 14536A-E7 | VGB6 | C57BL/6NTac | |
| 14536A-F12 | VGB6 | C57BL/6NTac | |
| 14536A-B8 | VGB6 | C57BL/6NTac | |
