| Gene | 1810009J06Rik |
| Chromosome | 6 |
| Deletion Start | 40,964,792 |
| Deletion End | 40,968,336 |
| Deletion Size | 3,545 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,964,921 | 40,964,941 | ATGTGCAAGGGGCTAAAAGCA |
| TUP | 40,964,944 | 40,964,968 | CAGTCAACACTATTTGCTACATTTA |
| TUR | 40,965,008 | 40,965,032 | CAGGATGCCCATTAAGTTCATTTGC |
| TDF | 40,968,007 | 40,968,037 | GCTTATAGAGACAGTATTAAAAATAAGGGGT |
| TDP | 40,968,121 | 40,968,144 | CGTGGAGTCCATTCTCGCACCTCA |
| TDR | 40,968,203 | 40,968,222 | GGATCTCTCCATTGCAGACG |
PCR Primers
| Primer | Sequence |
| SU | TCCTTTAATCTTTCTCCACTG |
| SD | AATGCTAATGCAAGCACTTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 216 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14539A-A12 | VGB6 | C57BL/6NTac | |
| 14539A-H6 | VGB6 | C57BL/6NTac | |
