| Gene | G6pd2 |
| Chromosome | 5 |
| Deletion Start | 61,808,887 |
| Deletion End | 61,810,526 |
| Deletion Size | 1,640 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,809,354 | 61,809,375 | CTGCATGAGTCAGACAGGCTTT |
| TUP | 61,809,378 | 61,809,404 | CCGCATCATAGTGGAGAAACCCTTCGG |
| TUR | 61,809,513 | 61,809,532 | CAAACCTCAGCACCATGAGG |
| TDF | 61,809,988 | 61,810,012 | GTGAGACTGCAATTCCGAGATATAC |
| TDP | 61,810,014 | 61,810,039 | AGGCGACATCTTCCACCAGAAGTGCA |
| TDR | 61,810,146 | 61,810,174 | CATTCTTGTATTTGTTGCCATAAGTTAGG |
PCR Primers
| Primer | Sequence |
| SU | GGTTATATGGCAGAATGATTTC |
| SD | CTCTGGGAAGTCTGGTTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 179 bp | + | + | - |
| TDF | TDR | 187 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14540A-F11 | VGB6 | C57BL/6NTac | |
| 14540A-C1 | VGB6 | C57BL/6NTac | |
