| Gene | Gcnt2 |
| Chromosome | 13 |
| Deletion Start | 40,860,358 |
| Deletion End | 40,918,797 |
| Deletion Size | 58,440 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 40,860,396 | 40,860,420 | TGCTGCCTTGATGCTTATGTTTATG |
| TUP | 40,860,439 | 40,860,468 | AAGAACTATCATTTCCCGAGGGCGGTTTCC |
| TUR | 40,860,478 | 40,860,498 | CAAGCAGACTTCCGCTAAGAC |
| TDF | 40,918,653 | 40,918,674 | CATCTACTTCGGCACAGCCTAC |
| TDP | 40,918,676 | 40,918,695 | TGGCCCTCACCCGGGACTTT |
| TDR | 40,918,708 | 40,918,729 | CAATGGCCCTTTCGTCATTCAG |
PCR Primers
| Primer | Sequence |
| SU | GGCCGAAAGGTCACTGATAG |
| SD | GGGTAGCACAAAACTGTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14541A-C7 | VGB6 | C57BL/6NTac | |
| 14541A-B6 | VGB6 | C57BL/6NTac | |
