| Gene | 1700019E19Rik |
| Chromosome | 12 |
| Deletion Start | 86,082,590 |
| Deletion End | 86,111,671 |
| Deletion Size | 29,082 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,083,088 | 86,083,107 | TTGCTTCCTCCCAGCCTATG |
| TUP | 86,083,108 | 86,083,131 | ACCGGCGTCCAGCCAGTAGATAGA |
| TUR | 86,083,212 | 86,083,232 | AGGTGGCCCAATCCTTCAGTG |
| TDF | 86,111,540 | 86,111,559 | CATGAACCTGGCGCTCTGTG |
| TDP | 86,111,563 | 86,111,585 | TTTGGAGGCACAGGCTGCATGAC |
| TDR | 86,111,587 | 86,111,608 | GTGTGAGAGAAGCCAGAGTGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGTTACAAATGTAGGCAG |
| SD | TGACTTGCCTTATTAAGGTTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 145 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14542A-B8 | VGB6 | C57BL/6NTac | |
| 14542A-D9 | VGB6 | C57BL/6NTac | |
| 14542A-F7 | VGB6 | C57BL/6NTac | |
| 14542A-H1 | VGB6 | C57BL/6NTac | |
| 14542A-E3 | VGB6 | C57BL/6NTac | |
| 14542A-E1 | VGB6 | C57BL/6NTac | |
