| Gene | Kif3c |
| Chromosome | 12 |
| Deletion Start | 3,365,984 |
| Deletion End | 3,403,009 |
| Deletion Size | 37,026 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,366,353 | 3,366,373 | GAGCACATCTTCACCCACATC |
| TUP | 3,366,375 | 3,366,396 | CCCGCTCCCAGAACCAGCAGTA |
| TUR | 3,366,441 | 3,366,461 | GCTCCTTGGATAGCAGGTCTC |
| TDF | 3,402,584 | 3,402,606 | AAAGTGCGAAAGTCGAGATCCTG |
| TDP | 3,402,610 | 3,402,635 | AGTACAACCGTGGTCAGAGTGAGCAC |
| TDR | 3,402,659 | 3,402,678 | GCCACTCAGACCCTCTTCAG |
PCR Primers
| Primer | Sequence |
| SU | CATTGTTGCCGAAGCTGTAG |
| SD | TGGATGGGTAAAGGAAGCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 109 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14545B-A5 | VGB6 | C57BL/6NTac | |
| 14545B-C8 | VGB6 | C57BL/6NTac | |
| 14545B-E2 | VGB6 | C57BL/6NTac | |
