| Gene | Socs1 |
| Chromosome | 16 |
| Deletion Start | 10,784,868 |
| Deletion End | 10,784,223 |
| Deletion Size | 646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,784,838 | 10,784,856 | CAGGTGGCAGCCGACAATG |
| TUP | 10,784,817 | 10,784,837 | CGATCTCCCCGGCAGCAGAGC |
| TUR | 10,784,775 | 10,784,794 | AGGACGAAGACGAGGACGAG |
| TDF | 10,784,522 | 10,784,542 | GAACTGCTTCTTCGCGCTCAG |
| TDP | 10,784,499 | 10,784,519 | TGAAGATGGCTTCGGGCCCCA |
| TDR | 10,784,424 | 10,784,443 | CGAAAAGGCAGTCGAAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CCTGAGCCCTGATCCTCC |
| SD | AATGAAGCCAGAGACCCTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 119 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14550A-A12 | VGB6 | C57BL/6NTac | |
| 14550A-H11 | VGB6 | C57BL/6NTac | |
| 14550A-H4 | VGB6 | C57BL/6NTac | |
| 14550A-E2 | VGB6 | C57BL/6NTac | |
| 14550A-F9 | VGB6 | C57BL/6NTac | |
