| Gene | Prm2 |
| Chromosome | 16 |
| Deletion Start | 10,791,995 |
| Deletion End | 10,791,475 |
| Deletion Size | 521 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,791,818 | 10,791,838 | GCTGCTCTCGTAAGAGGCTAC |
| TUP | 10,791,792 | 10,791,816 | TAGGATCCACAAGAGGCGTCGGTCA |
| TUR | 10,791,769 | 10,791,789 | GAGTGTCTCCTCCGCCTTCTG |
| TDF | 10,791,568 | 10,791,587 | AGGAGGCACCATCACTAAGC |
| TDP | 10,791,538 | 10,791,561 | AGGCCTGTCCATTCTGCCTGGAGC |
| TDR | 10,791,500 | 10,791,520 | TGGGCAGGTGACTATTCCTTG |
PCR Primers
| Primer | Sequence |
| SU | TAATGCACATGGAGTGGGTG |
| SD | AACTCATGTGCTGGGAAGATG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14552A-A3 | VGB6 | C57BL/6NTac | |
| 14552A-D2 | VGB6 | C57BL/6NTac | |
| 14552A-D10 | VGB6 | C57BL/6NTac | |
| 14552A-G5 | VGB6 | C57BL/6NTac | |
| 14552A-H9 | VGB6 | C57BL/6NTac | |
| 14552A-H11 | VGB6 | C57BL/6NTac | |
