| Gene | Thap11 |
| Chromosome | 8 |
| Deletion Start | 105,855,364 |
| Deletion End | 105,856,365 |
| Deletion Size | 1,002 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 105,855,387 | 105,855,408 | GGGCTGCTACAACAATTCACAC |
| TUP | 105,855,409 | 105,855,432 | CGGGACAAGGCGCTGCACTTCTAC |
| TUR | 105,855,435 | 105,855,456 | CAACTCAGCGTCCTTGGGAAAC |
| TDF | 105,856,136 | 105,856,155 | TGGCCCTGATGGAAGTGAAG |
| TDP | 105,856,159 | 105,856,184 | AAGGAGATGAAGGGTAGCATCCGCCA |
| TDR | 105,856,190 | 105,856,208 | AGCTTGGCCTCGGTGAGAC |
PCR Primers
| Primer | Sequence |
| SU | AACAGGATATCGCGGTGTCC |
| SD | TCACAGACTGCACCTGAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14553A-F5 | VGB6 | C57BL/6NTac | |
| 14553A-E12 | VGB6 | C57BL/6NTac | |
| 14553A-E5 | VGB6 | C57BL/6NTac | |
| 14553A-A5 | VGB6 | C57BL/6NTac | |
| 14553A-G10 | VGB6 | C57BL/6NTac | |
