| Gene | Krit1 |
| Chromosome | 5 |
| Deletion Start | 3,806,470 |
| Deletion End | 3,840,952 |
| Deletion Size | 34,483 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,806,779 | 3,806,800 | GGGCTGAGATGTAAACCAGTTG |
| TUP | 3,806,802 | 3,806,827 | CAGAGGGCTTGGCTGGCATAAATGTC |
| TUR | 3,806,831 | 3,806,851 | AGGCAGTGCTGAGAATCAAAC |
| TDF | 3,840,192 | 3,840,213 | CCAAGTAAGCTGGTGGGTAAAC |
| TDP | 3,840,219 | 3,840,248 | AACTGAACCAGAGAAAGATGGCTCTGAACC |
| TDR | 3,840,256 | 3,840,276 | ACTCCCTTTGCCTGGCCTCAG |
PCR Primers
| Primer | Sequence |
| SU | GCCTCAAATTCTTGATTCTTGA |
| SD | AATATAATCCAGACTGCTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 73 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14555A-E2 | VGB6 | C57BL/6NTac | |
| 14555A-H5 | VGB6 | C57BL/6NTac | |
| 14555A-F11 | VGB6 | C57BL/6NTac | |
