| Gene | Ccdc86 |
| Chromosome | 19 |
| Deletion Start | 10,949,230 |
| Deletion End | 10,943,160 |
| Deletion Size | 6,071 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,948,684 | 10,948,702 | GATCTGGGCCTGGAGTCAC |
| TUP | 10,948,660 | 10,948,682 | TGCAGGACAGACAGAGTCGAGCC |
| TUR | 10,948,622 | 10,948,644 | AGGTAATTTGGATGGCTGCTCTC |
| TDF | 10,943,825 | 10,943,846 | GGCCATTCTGCTTGTATGTGAC |
| TDP | 10,943,804 | 10,943,824 | TTCTGAGCGGGAAGGCACCCA |
| TDR | 10,943,781 | 10,943,803 | GGATGACTGTATGGGCCAAGATG |
PCR Primers
| Primer | Sequence |
| SU | TTGCAAGAACTCGCAGTTTG |
| SD | TGGGAAACAAGAATTCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 66 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14559A-A2 | VGB6 | C57BL/6NTac | |
| 14559A-A7 | VGB6 | C57BL/6NTac | |
| 14559A-A10 | VGB6 | C57BL/6NTac | |
| 14559A-C5 | VGB6 | C57BL/6NTac | |
| 14559A-E3 | VGB6 | C57BL/6NTac | |
| 14559A-F11 | VGB6 | C57BL/6NTac | |
