| Gene | Bcor |
| Chromosome | X |
| Deletion Start | 12,061,604 |
| Deletion End | 12,037,610 |
| Deletion Size | 23,995 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 12,061,352 | 12,061,372 | AGCTGGTGGCCCTTGATTTAC |
| TUP | 12,061,325 | 12,061,348 | TTTAACAGGCAGCCTGGTTCAGCG |
| TUR | 12,061,265 | 12,061,284 | GGACGAAAGCGCACTGAAAG |
| TDF | 12,037,722 | 12,037,743 | TCCTGAAAGCAAGGAGCTGTTG |
| TDP | 12,037,693 | 12,037,719 | TCTGGTGGAATTCACCAATGAGCTCCA |
| TDR | 12,037,645 | 12,037,664 | CCCGTGTCACTAGGGTGGAG |
PCR Primers
| Primer | Sequence |
| SU | ACCACCTCACTGTTTCTGCC |
| SD | ATTTCAAAGCATGTGTACACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 108 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14562A-E9 | VGB6 | C57BL/6NTac | |
| 14562B-G12 | VGB6 | C57BL/6NTac | |
| 14562C-A1 | VGB6 | C57BL/6NTac | |
| 14562C-A3 | VGB6 | C57BL/6NTac | |
| 14562C-A12 | VGB6 | C57BL/6NTac | |
