| Gene | Palb2 |
| Chromosome | 7 |
| Deletion Start | 122,132,764 |
| Deletion End | 122,107,429 |
| Deletion Size | 25,336 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 122,132,683 | 122,132,701 | GGGTGACCCAGGGCTTTAG |
| TUP | 122,132,647 | 122,132,675 | TTTCAAACCTAGTTCCTGTATCCGAGGCA |
| TUR | 122,132,619 | 122,132,638 | CTCGTGGAGGCTGTAGGTAG |
| TDF | 122,107,639 | 122,107,660 | GGGATTTGCTTCTGGGTCACTG |
| TDP | 122,107,613 | 122,107,637 | ACGGCTCTCCTCCCACCTGTCTCTG |
| TDR | 122,107,579 | 122,107,602 | GTACCCGACCATTTAACCAAAGAC |
PCR Primers
| Primer | Sequence |
| SU | CGGTGATTCCAGCTACTTCC |
| SD | CCACTGAGTTATTGCACTGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14579A-E1 | VGB6 | C57BL/6NTac | |
| 14579B-G1 | VGB6 | C57BL/6NTac | |
| 14579B-G5 | VGB6 | C57BL/6NTac | |
| 14579C-F3 | VGB6 | C57BL/6NTac | |
