| Gene | Pou1f1 |
| Chromosome | 16 |
| Deletion Start | 65,520,632 |
| Deletion End | 65,533,982 |
| Deletion Size | 13,351 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,520,691 | 65,520,709 | TGCCCTGCCTCTGAGAATG |
| TUP | 65,520,712 | 65,520,734 | CCACAGTGCCGCTGAGTGTCTCC |
| TUR | 65,520,758 | 65,520,779 | GGTCAGTACCTGTGGACATCAC |
| TDF | 65,533,809 | 65,533,827 | AGCACAGCAAGCCTTCCTC |
| TDP | 65,533,829 | 65,533,856 | CAGGAGATCATGCGGATGGCTGAAGAAC |
| TDR | 65,533,883 | 65,533,900 | TCGGTTGCAGAACCACAC |
PCR Primers
| Primer | Sequence |
| SU | TTTCTGTCTTTGCCTGCTCC |
| SD | TGAGACAAAAACTGCAGGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14581A-C4 | VGB6 | C57BL/6NTac | |
| 14581B-C2 | VGB6 | C57BL/6NTac | |
| 14581C-H6 | VGB6 | C57BL/6NTac | |
| 14581C-C8 | VGB6 | C57BL/6NTac | |
