| Gene | Wdr20b |
| Chromosome | 12 |
| Deletion Start | 65,225,768 |
| Deletion End | 65,227,476 |
| Deletion Size | 1,709 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 65,225,870 | 65,225,889 | AACCGGGTGCCCTTCAACTC |
| TUP | 65,225,891 | 65,225,915 | CAGGGATCCAATCCTGTCCGCGTCT |
| TUR | 65,225,938 | 65,225,956 | GCGGTTGCCATTACCAGTC |
| TDF | 65,226,805 | 65,226,829 | GTCTAGACTATCCAAACAGAACTCG |
| TDP | 65,226,831 | 65,226,856 | CAGACAGCCGCCCTGTAAGTGTTACG |
| TDR | 65,226,959 | 65,226,980 | CTGTGGCATTCATAACGTTTGC |
PCR Primers
| Primer | Sequence |
| SU | GGATGTCCTCCCTGGTATTTC |
| SD | TGACCAGGTATACAACAATCTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 176 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14588A-E4 | VGB6 | C57BL/6NTac | |
| 14588A-H11 | VGB6 | C57BL/6NTac | |
