| Gene | Cacna1s |
| Chromosome | 1 |
| Deletion Start | 136,052,904 |
| Deletion End | 136,077,705 |
| Deletion Size | 24,802 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,053,230 | 136,053,249 | GTCTCCGCCACAATGGAAAG |
| TUP | 136,053,254 | 136,053,278 | TTCTCGGAGCCTGAGGACAACTCTC |
| TUR | 136,053,298 | 136,053,320 | CCACAAGATGCTCAATAGCTCAG |
| TDF | 136,077,138 | 136,077,156 | GGTCCCACCTGCAATGAAC |
| TDP | 136,077,157 | 136,077,177 | TGGGCCCTCCCACATCGATCA |
| TDR | 136,077,203 | 136,077,223 | AAGACTGGGCTGTAGGCAATC |
PCR Primers
| Primer | Sequence |
| SU | TGTGAAGCCTTCCTGTCTCC |
| SD | ACTTCCTTCCTGCAGGCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14596A-D6 | VGB6 | C57BL/6NTac | |
| 14596B-E12 | VGB6 | C57BL/6NTac | |
