| Gene | 1700088E04Rik |
| Chromosome | 15 |
| Deletion Start | 79,140,980 |
| Deletion End | 79,135,096 |
| Deletion Size | 5,885 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,140,960 | 79,140,979 | CTTCCCAGGAAAGGGTGGAC |
| TUP | 79,140,938 | 79,140,959 | TCGGTGACCAAAGGAACCGGGT |
| TUR | 79,140,902 | 79,140,920 | CCGGCGTGTAACCTGCTTG |
| TDF | 79,135,928 | 79,135,949 | GGTGCTTGTTCTCGGTTGTTTC |
| TDP | 79,135,904 | 79,135,927 | CACTGCTAGCCTAACACGCTCTGC |
| TDR | 79,135,879 | 79,135,900 | CCCACACTTCTTTGGCTGTTTC |
PCR Primers
| Primer | Sequence |
| SU | GTGCTCACTTGTCCTCGTTG |
| SD | TTGCCCAAGGCTACAAGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14600A-G5 | VGB6 | C57BL/6NTac | |
| 14600A-G6 | VGB6 | C57BL/6NTac | |
