| Gene | Akr1c14 |
| Chromosome | 13 |
| Deletion Start | 4,059,625 |
| Deletion End | 4,088,987 |
| Deletion Size | 29,363 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 4,059,791 | 4,059,813 | GCTTCAGGCTCACATTAGACATC |
| TUP | 4,059,818 | 4,059,840 | CTGCAGATTACAGCTATTCATGT |
| TUR | 4,059,856 | 4,059,879 | GTCTCCAAGGAACAGTAACAAGTC |
| TDF | 4,088,003 | 4,088,025 | CAGACCTCTCAGTTCCTTTTGCT |
| TDP | 4,088,028 | 4,088,049 | ACATTTGTTCAGCACTCAAAGC |
| TDR | 4,088,068 | 4,088,092 | TTCTATTCACCTCTCAAAGATCAGC |
PCR Primers
| Primer | Sequence |
| SU | CCAGAGCCCAGTAGCTGAAG |
| SD | GCAGGCAAGAATCTTTAGAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 89 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14618D-B2 | VGB6 | C57BL/6NTac | |
| 14618D-B5 | VGB6 | C57BL/6NTac | |
| 14618E-B2 | VGB6 | C57BL/6NTac | |
