| Gene | Mex3a |
| Chromosome | 3 |
| Deletion Start | 88,536,153 |
| Deletion End | 88,537,277 |
| Deletion Size | 1,125 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,536,462 | 88,536,482 | GAGGAGATCGAGACGCACATC |
| TUP | 88,536,487 | 88,536,509 | TGCGCACCGGCAAGATTCTTGAA |
| TUR | 88,536,512 | 88,536,529 | AAGTCGCCGTCGCTGTTG |
| TDF | 88,537,019 | 88,537,038 | GGACTGCATGGTGTGCTTTG |
| TDP | 88,537,039 | 88,537,063 | AGAGTGAAGTGACAGCAGCCCTGGT |
| TDR | 88,537,099 | 88,537,118 | CCTCTCGCAGATGCGTACTG |
PCR Primers
| Primer | Sequence |
| SU | TGAGCAAAGACCATGAGCAG |
| SD | ACAGATGGCAAGTCTCCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 100 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14620A-H2 | VGB6 | C57BL/6NTac | |
| 14620A-E12 | VGB6 | C57BL/6NTac | |
| 14620A-F10 | VGB6 | C57BL/6NTac | |
| 14620A-G9 | VGB6 | C57BL/6NTac | |
| 14620A-C3 | VGB6 | C57BL/6NTac | |
