| Gene | Mov10l1 |
| Chromosome | 15 |
| Deletion Start | 88,985,577 |
| Deletion End | 89,011,415 |
| Deletion Size | 25,839 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,985,730 | 88,985,750 | CACCCTGAGTCACCACATTTG |
| TUP | 88,985,752 | 88,985,776 | TAGCTTCTCAGTCATGGCCTAGCCA |
| TUR | 88,985,781 | 88,985,803 | TCACAGCCGATTTCACTAGATTC |
| TDF | 89,011,005 | 89,011,025 | TCTGAAGGCTTTGCAGCTTTG |
| TDP | 89,011,027 | 89,011,046 | TGAGGGCCTCCCAGCTGCAT |
| TDR | 89,011,062 | 89,011,080 | TGCTCCCTTGTCAGTGCTC |
PCR Primers
| Primer | Sequence |
| SU | TTTAATGGTAGTGGGATTTGGG |
| SD | TACCAGGAGTATAATGCAGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14621A-D7 | VGB6 | C57BL/6NTac | |
| 14621A-H5 | VGB6 | C57BL/6NTac | |
| 14621A-C4 | VGB6 | C57BL/6NTac | |
| 14621A-D9 | VGB6 | C57BL/6NTac | |
| 14621A-E11 | VGB6 | C57BL/6NTac | |
