| Gene | Igf1r |
| Chromosome | 7 |
| Deletion Start | 68,003,815 |
| Deletion End | 68,004,350 |
| Deletion Size | 536 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 68,003,878 | 68,003,898 | ATCGAGGGCTTCCTCCACATC |
| TUP | 68,003,900 | 68,003,921 | TGCTCATCTCCAAGGCCGAGGA |
| TUR | 68,003,971 | 68,003,989 | CAGCGACTCGGAAGAGCAG |
| TDF | 68,004,261 | 68,004,282 | TCCAGGGACATTGGAGGAGAAG |
| TDP | 68,004,283 | 68,004,310 | CCCATGTGTGAGAAGACCACCATCAACA |
| TDR | 68,004,313 | 68,004,333 | CCAGCAGCGGTAGTTGTACTC |
PCR Primers
| Primer | Sequence |
| SU | TGTAGCATGAACTCTGCACAC |
| SD | TAACCAGACTTGACAGCAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14637A-E8 | VGB6 | C57BL/6NTac | |
| 14637A-E2 | VGB6 | C57BL/6NTac | |
| 14637A-E3 | VGB6 | C57BL/6NTac | |
| 14637A-G11 | VGB6 | C57BL/6NTac | |
| 14637A-G12 | VGB6 | C57BL/6NTac | |
| 14637A-D11 | VGB6 | C57BL/6NTac | |
