| Gene | Slc7a4 |
| Chromosome | 16 |
| Deletion Start | 17,575,930 |
| Deletion End | 17,573,273 |
| Deletion Size | 2,658 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 17,575,837 | 17,575,858 | GAGTCTAGCATGGAGACATCAC |
| TUP | 17,575,815 | 17,575,834 | CGGCGCTGCCTGTCCACACT |
| TUR | 17,575,791 | 17,575,811 | CCCACACCCAGTAGAGTCAAG |
| TDF | 17,573,338 | 17,573,357 | AGCGGTAGCCTGGAGGAAAC |
| TDP | 17,573,316 | 17,573,337 | TGTGCAAGCTGTGCAGCCCTCT |
| TDR | 17,573,293 | 17,573,312 | GACTCCCGGACTGGTGATTG |
PCR Primers
| Primer | Sequence |
| SU | CTCCCATTTCTTCTTGCTCG |
| SD | GGCAGGTGAGAACTCAGAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 65 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14640A-D4 | VGB6 | C57BL/6NTac | |
| 14640A-D10 | VGB6 | C57BL/6NTac | |
| 14640A-H4 | VGB6 | C57BL/6NTac | |
| 14640A-E3 | VGB6 | C57BL/6NTac | |
| 14640A-G12 | VGB6 | C57BL/6NTac | |
| 14640A-D12 | VGB6 | C57BL/6NTac | |
