| Gene | Cdkn1a |
| Chromosome | 17 |
| Deletion Start | 29,098,411 |
| Deletion End | 29,099,454 |
| Deletion Size | 1,044 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 29,098,733 | 29,098,751 | TGGCCTTGTCGCTGTCTTG |
| TUP | 29,098,754 | 29,098,775 | CTCTGGTGTCTGAGCGGCCTGA |
| TUR | 29,098,861 | 29,098,880 | GGCCTCTTGCAGGATCTTTC |
| TDF | 29,099,109 | 29,099,129 | TGAGGGCACTTACTCTTGCTG |
| TDP | 29,099,130 | 29,099,151 | AGGGCCTGATTCCCAGCACCGG |
| TDR | 29,099,158 | 29,099,182 | GCCCAGAGTTAGTTATAGGAAGTTG |
PCR Primers
| Primer | Sequence |
| SU | TGGACCTAGCAATTCACACG |
| SD | GTGAAACGGATGCAGATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 148 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14645C-G10 | VGB6 | C57BL/6NTac | |
| 14645D-C2 | VGB6 | C57BL/6NTac | |
| 14645D-E11 | VGB6 | C57BL/6NTac | |
| 14645D-H4 | VGB6 | C57BL/6NTac | |
| 14645D-B2 | VGB6 | C57BL/6NTac | |
