| Gene | BC048609 |
| Chromosome | 19 |
| Deletion Start | 6,080,146 |
| Deletion End | 6,080,791 |
| Deletion Size | 646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,080,199 | 6,080,220 | TGTAGCCGCACTTATACTCTTC |
| TUP | 6,080,226 | 6,080,255 | ACACATGGGTGTCTTTGCTAGTGATGTTCA |
| TUR | 6,080,272 | 6,080,292 | GCTCATGTGGTCGTAGTTGTG |
| TDF | 6,080,593 | 6,080,615 | TGTTGAGCTGATGATGGATTGTC |
| TDP | 6,080,616 | 6,080,641 | TCCTCTACCCAGTCCTCAATGGAGCC |
| TDR | 6,080,644 | 6,080,664 | AGGCACAGGCGGTTGAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | ACAGTGTAGAGGATTGAATGG |
| SD | GCCACTGACTCTGCTCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14646A-E5 | VGB6 | C57BL/6NTac | |
| 14646C-G9 | VGB6 | C57BL/6NTac | |
