| Gene | Rb1cc1 |
| Chromosome | 1 |
| Deletion Start | 6,230,006 |
| Deletion End | 6,250,160 |
| Deletion Size | 20,155 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 6,230,183 | 6,230,201 | CCAAAGCCCAAGCCGAAAG |
| TUP | 6,230,219 | 6,230,248 | TGCTCTTGTAATGGAAGGTTAGGGAATCCC |
| TUR | 6,230,253 | 6,230,275 | CAAACTAGGCTAGACCGTCTGTC |
| TDF | 6,249,332 | 6,249,352 | CACATCAGGCACACACAGGAG |
| TDP | 6,249,354 | 6,249,377 | TTGAGAAAGTTATGACAGACCACA |
| TDR | 6,249,509 | 6,249,535 | TTCAACCTCTAACTTACATCTCATGTC |
PCR Primers
| Primer | Sequence |
| SU | CGTTGAATTTCTCCTCCAGC |
| SD | CAATAGTGAGGACCATTCTGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 204 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14654A-D9 | VGB6 | C57BL/6NTac | |
| 14654A-E8 | VGB6 | C57BL/6NTac | |
| 14654A-F6 | VGB6 | C57BL/6NTac | |
| 14654A-G6 | VGB6 | C57BL/6NTac | |
| 14654A-G11 | VGB6 | C57BL/6NTac | |
| 14654A-D12 | VGB6 | C57BL/6NTac | |
