| Gene | Gspt2 |
| Chromosome | X |
| Deletion Start | 94,636,260 |
| Deletion End | 94,638,317 |
| Deletion Size | 2,058 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 94,636,430 | 94,636,449 | GCTCAACATCCACGCCAAAC |
| TUP | 94,636,454 | 94,636,474 | CGTGCCTAGCGTAAGCGCAGC |
| TUR | 94,636,476 | 94,636,495 | AGGAAGGACGGCACGAACTC |
| TDF | 94,637,777 | 94,637,799 | TCTTCCAGGCTTCATACTTTGTG |
| TDP | 94,637,802 | 94,637,828 | CCCAGTAATCTTTGCCATTCCGGACGC |
| TDR | 94,637,859 | 94,637,881 | CCTGGGCAGATGATAGACTTGTG |
PCR Primers
| Primer | Sequence |
| SU | GACAAACCTACGGATGGCAG |
| SD | CTGAAGTTTCAAACGCAACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 105 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14664A-F3 | VGB6 | C57BL/6NTac | |
| 14664A-G8 | VGB6 | C57BL/6NTac | |
