| Gene | Prokr2 |
| Chromosome | 2 |
| Deletion Start | 132,381,617 |
| Deletion End | 132,373,345 |
| Deletion Size | 8,273 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 132,381,381 | 132,381,400 | CCCTCGCCCGCTACAAGAAG |
| TUP | 132,381,356 | 132,381,380 | CTGCGCAACCTTACCAACCTCCTCA |
| TUR | 132,381,318 | 132,381,337 | GATCGCCACCAGGAAGTCAG |
| TDF | 132,373,832 | 132,373,850 | GGGTCCCGTGGTCACTATG |
| TDP | 132,373,806 | 132,373,829 | CCTGTGCTATGCCAGGATCTCCCA |
| TDR | 132,373,757 | 132,373,774 | GCGGATTTGCTCCGTCTG |
PCR Primers
| Primer | Sequence |
| SU | TCATTCTGTGACCAGCAAGC |
| SD | TGACTCTGGCTAGTCATTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 83 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14667A-A1 | VGB6 | C57BL/6NTac | |
| 14667B-B2 | VGB6 | C57BL/6NTac | |
| 14667B-C6 | VGB6 | C57BL/6NTac | |
| 14667C-E3 | VGB6 | C57BL/6NTac | |
