| Gene | Eif2ak3 |
| Chromosome | 6 |
| Deletion Start | 70,844,729 |
| Deletion End | 70,858,512 |
| Deletion Size | 13,784 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 70,845,262 | 70,845,285 | CACTCCCATTCCCAAATACTTGAC |
| TUP | 70,845,290 | 70,845,316 | TCTGTCCTGATTCTGTTGCGACAGGTA |
| TUR | 70,845,366 | 70,845,384 | CTCGACCAGGAGCCACAAC |
| TDF | 70,857,628 | 70,857,650 | GTCACCAGCAGCTAAACTCTTCC |
| TDP | 70,857,657 | 70,857,684 | AAGCTAGTTTCCTTTGTGAGGGTCAGCA |
| TDR | 70,857,702 | 70,857,723 | ACCAGACTGTCCCATCCATCAC |
PCR Primers
| Primer | Sequence |
| SU | AATGTTGACAACAAGGAACGC |
| SD | TTGGAGAGCCGAAGATGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 123 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14677A-C7 | VGB6 | C57BL/6NTac | |
| 14677B-B7 | VGB6 | C57BL/6NTac | |
