| Gene | Pld6 |
| Chromosome | 11 |
| Deletion Start | 59,787,632 |
| Deletion End | 59,784,975 |
| Deletion Size | 2,658 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 59,786,769 | 59,786,789 | TGGAAAGTGGACCCTACAGTG |
| TUP | 59,786,742 | 59,786,768 | TGGCGAATACTTGCTTCTCAGCGATCA |
| TUR | 59,786,708 | 59,786,726 | GTAGGCGGGCCAGACATAC |
| TDF | 59,785,262 | 59,785,281 | AGTCCCTGGGCATCCTTGTC |
| TDP | 59,785,236 | 59,785,261 | TGGGTGGGTGACATAAAGGCTACCTT |
| TDR | 59,785,206 | 59,785,225 | GTCGTGCCGTACCTGTATCC |
PCR Primers
| Primer | Sequence |
| SU | GCTAGCTTGGGAAAGCTGTG |
| SD | CTAGTGATGCCCAGGAGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14689A-D6 | VGB6 | C57BL/6NTac | |
| 14689A-F6 | VGB6 | C57BL/6NTac | |
| 14689A-C7 | VGB6 | C57BL/6NTac | |
| 14689A-C12 | VGB6 | C57BL/6NTac | |
| 14689A-G7 | VGB6 | C57BL/6NTac | |
| 14689A-E8 | VGB6 | C57BL/6NTac | |
