| Gene | Tbcc |
| Chromosome | 17 |
| Deletion Start | 46,890,693 |
| Deletion End | 46,891,728 |
| Deletion Size | 1,036 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,891,046 | 46,891,065 | GCAGGCTGTCCTAACGGAAC |
| TUP | 46,891,068 | 46,891,091 | CGCCAGGAGTTGCAGCCCAAGAAG |
| TUR | 46,891,093 | 46,891,110 | GTGCCTTGAAGGCGAAGC |
| TDF | 46,891,502 | 46,891,520 | CAGCAGGGCTATCGTGGAG |
| TDP | 46,891,522 | 46,891,544 | ACTGCAGCGGCATCCAGTTTGCC |
| TDR | 46,891,578 | 46,891,597 | AAACCGGAGTCCTGGAAGTC |
PCR Primers
| Primer | Sequence |
| SU | CCCACAATCCCGGATATAAG |
| SD | TTTAAACCGTCCGCTAATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 96 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14691A-A9 | VGB6 | C57BL/6NTac | |
| 14691A-G2 | VGB6 | C57BL/6NTac | |
| 14691A-B6 | VGB6 | C57BL/6NTac | |
| 14691A-C9 | VGB6 | C57BL/6NTac | |
| 14691A-F9 | VGB6 | C57BL/6NTac | |
| 14691A-B1 | VGB6 | C57BL/6NTac | |
