| Gene | Cnnm2 |
| Chromosome | 19 |
| Deletion Start | 46,761,776 |
| Deletion End | 46,763,389 |
| Deletion Size | 1,614 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 46,762,457 | 46,762,477 | GCCGAGACCACCTGGATTTAC |
| TUP | 46,762,478 | 46,762,502 | CACGACGGTGAGGACACCAAGATGA |
| TUR | 46,762,522 | 46,762,540 | CCAGAAGGGCAGCAAGAAC |
| TDF | 46,763,018 | 46,763,038 | GAGATGCTCCGGGTTACTGAC |
| TDP | 46,763,045 | 46,763,072 | AACGACCTCGTTAAGGAAGAGCTGAACA |
| TDR | 46,763,101 | 46,763,119 | CACGTCCTCCACCGTCTTG |
PCR Primers
| Primer | Sequence |
| SU | AAACTTGGCATGCAGAGGAG |
| SD | ACACTGGAATTACAAAGCCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 102 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14695A-D6 | VGB6 | C57BL/6NTac | |
| 14695A-F5 | VGB6 | C57BL/6NTac | |
