| Gene | Ina |
| Chromosome | 19 |
| Deletion Start | 47,014,758 |
| Deletion End | 47,023,725 |
| Deletion Size | 8,968 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 47,015,574 | 47,015,591 | TCCCTGGCCGCTAAGAAC |
| TUP | 47,015,595 | 47,015,617 | CAGTCCGCCGAGGAGTGGTACAA |
| TUR | 47,015,621 | 47,015,641 | TCGTTTAGGTTGGCGAACTTG |
| TDF | 47,023,345 | 47,023,367 | GAAGGTGAAGAGACGCGGTTTAG |
| TDP | 47,023,368 | 47,023,390 | CACCGGCGGGTTAAGCATCTCGG |
| TDR | 47,023,413 | 47,023,434 | TCTGGGAGGGAGCAAATAACTG |
PCR Primers
| Primer | Sequence |
| SU | AACGCCATCAAAGAGCTGAG |
| SD | TGAAGAGTGGAAGGCATGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 90 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14700A-A9 | VGB6 | C57BL/6NTac | |
| 14700A-F9 | VGB6 | C57BL/6NTac | |
| 14700A-H12 | VGB6 | C57BL/6NTac | |
| 14700A-H10 | VGB6 | C57BL/6NTac | |
| 14700A-F12 | VGB6 | C57BL/6NTac | |
