| Gene | Tcof1 |
| Chromosome | 18 |
| Deletion Start | 60,848,855 |
| Deletion End | 60,814,361 |
| Deletion Size | 34,495 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 60,848,390 | 60,848,408 | GGGAACACGGAGACGAAGG |
| TUP | 60,848,355 | 60,848,384 | TAAGTTGATGATCTAGAATGCAGGGCGTGC |
| TUR | 60,848,304 | 60,848,321 | GCACCACGGGTTCAAGTC |
| TDF | 60,815,205 | 60,815,227 | AGTTCTGAGGCCAGATTGTGTAG |
| TDP | 60,815,177 | 60,815,203 | CCACAGTCAGTAAGAGTGGAGCCTGGC |
| TDR | 60,815,124 | 60,815,144 | CAGCTCGCGGCTCTTAACATC |
PCR Primers
| Primer | Sequence |
| SU | ACACGCCTTCGAGCCAATG |
| SD | AATTCACGCTGGCCAGAGGT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 105 bp | + | + | - |
| TDF | TDR | 104 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14701A-F5 | VGB6 | C57BL/6NTac | |
| 14701A-H1 | VGB6 | C57BL/6NTac | |
| 14701A-H5 | VGB6 | C57BL/6NTac | |
| 14701A-G4 | VGB6 | C57BL/6NTac | |
| 14701A-B4 | VGB6 | C57BL/6NTac | |
