| Gene | Akap10 |
| Chromosome | 11 |
| Deletion Start | 61,930,073 |
| Deletion End | 61,900,314 |
| Deletion Size | 29,760 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 61,929,822 | 61,929,840 | GGACTCCGCAGGGAAGAAG |
| TUP | 61,929,800 | 61,929,820 | TGGCCTCCTCAAGCGAGGTCG |
| TUR | 61,929,775 | 61,929,794 | TTTGGCCCAAAGCGACAATG |
| TDF | 61,901,123 | 61,901,146 | AGCCCACTATGGCACTTAGATTTG |
| TDP | 61,901,092 | 61,901,115 | AACCTATGGCTTCTGGGAGCAGCA |
| TDR | 61,901,061 | 61,901,083 | GCTGTATGCCTTACCTGCTGTAG |
PCR Primers
| Primer | Sequence |
| SU | GGAGGTTCAGCAAAGAGACG |
| SD | GTCTGTGAAACCCTTCTGAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 66 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14707A-B12 | VGB6 | C57BL/6NTac | |
| 14707B-H1 | VGB6 | C57BL/6NTac | |
| 14707B-G9 | VGB6 | C57BL/6NTac | |
