| Gene | Hnrnph3 |
| Chromosome | 10 |
| Deletion Start | 63,019,501 |
| Deletion End | 63,015,618 |
| Deletion Size | 3,884 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 63,019,208 | 63,019,231 | AGAGAATGTGTAGGCTCTGTGAAC |
| TUP | 63,019,159 | 63,019,188 | AACTGAGAGTAACTGATGACATGGGCTGTG |
| TUR | 63,019,137 | 63,019,157 | AACTGTGCTCCCAATGGTAAC |
| TDF | 63,016,512 | 63,016,533 | CCGCTCAATCCAATACGTGTTC |
| TDP | 63,016,484 | 63,016,509 | ATTGACATTGGAGCCGATGGCAGAGC |
| TDR | 63,016,440 | 63,016,462 | CTGCATCTTCATGTGTCACAAAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAACTTGTGGGTCTTTAGGTC |
| SD | TGTTCCTGAAACCACCTGAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 95 bp | + | + | - |
| TDF | TDR | 94 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14708A-B7 | VGB6 | C57BL/6NTac | |
| 14708A-H7 | VGB6 | C57BL/6NTac | |
| 14708A-E8 | VGB6 | C57BL/6NTac | |
| 14708A-E4 | VGB6 | C57BL/6NTac | |
| 14708A-E9 | VGB6 | C57BL/6NTac | |
| 14708A-A8 | VGB6 | C57BL/6NTac | |
