| Gene | Camk2n2 |
| Chromosome | 16 |
| Deletion Start | 20,621,191 |
| Deletion End | 20,620,033 |
| Deletion Size | 1,159 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,620,689 | 20,620,710 | GGCTGAGATGAGTAGGTGGATG |
| TUP | 20,620,668 | 20,620,688 | TTACTGGAGGGCACCGAGGCC |
| TUR | 20,620,625 | 20,620,646 | TGAGTCCCCTCCCAATACAAAC |
| TDF | 20,620,451 | 20,620,472 | CATTGGGAGTCTTCGGAAGAGG |
| TDP | 20,620,422 | 20,620,446 | TTCCCACATAGGGAGGTTTGGGTGC |
| TDR | 20,620,399 | 20,620,418 | GACCCTACCTGCTACCAAGC |
PCR Primers
| Primer | Sequence |
| SU | ATCACCGGACTCCCTCGAA |
| SD | TGGGTAGGAAGCTCATGCAA |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 86 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14709A-E6 | VGB6 | C57BL/6NTac | |
| 14709A-E5 | VGB6 | C57BL/6NTac | |
| 14709B-C7 | VGB6 | C57BL/6NTac | |
| 14709B-D4 | VGB6 | C57BL/6NTac | |
| 14709B-G4 | VGB6 | C57BL/6NTac | |
