| Gene | Ascl1 |
| Chromosome | 10 |
| Deletion Start | 87,493,085 |
| Deletion End | 87,492,367 |
| Deletion Size | 719 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,492,852 | 87,492,870 | TCACAAGTCAGCGGCCAAG |
| TUP | 87,492,829 | 87,492,850 | AGGTCAAGCGCCAGCGCTCGTC |
| TUR | 87,492,802 | 87,492,821 | CGTTTGCAGCGCATCAGTTC |
| TDF | 87,492,466 | 87,492,485 | TCTCCGGTCTCGTCCTACTC |
| TDP | 87,492,442 | 87,492,463 | CCGACGAGGGATCCTACGACCC |
| TDR | 87,492,413 | 87,492,431 | CCAGCAGCTCTTGTTCCTC |
PCR Primers
| Primer | Sequence |
| SU | ATCCCAGAAGCAGGCTCAAG |
| SD | GGCTTGGAGAATCTGTCAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14715A-A6 | VGB6 | C57BL/6NTac | |
| 14715A-B6 | VGB6 | C57BL/6NTac | |
| 14715A-B11 | VGB6 | C57BL/6NTac | |
| 14715A-G5 | VGB6 | C57BL/6NTac | |
| 14715A-H3 | VGB6 | C57BL/6NTac | |
