14716 Tmc1 Status: ES cell colonies screened / QC no positives
| Gene | Tmc1 |
| Chromosome | 19 |
| Deletion Start | 20,940,905 |
| Deletion End | 20,913,507 |
| Deletion Size | 27,399 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 19
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 20,940,349 | 20,940,371 | GCGGAGTGAGGTTCTTTGAACTG |
| TUP | 20,940,320 | 20,940,346 | ACAGGCTCTTTGGATTGACCTGGAACA |
| TUR | 20,940,296 | 20,940,313 | ATCTTGGGCAAGGGCATC |
| TDF | 20,914,110 | 20,914,131 | TTGTACCTGTGAGAGTCTGAGG |
| TDP | 20,914,078 | 20,914,106 | CACCAAATAGTAAACGGCAGTCGTCTGGG |
| TDR | 20,914,033 | 20,914,054 | GGAGGAAGCTAGGACTGGTAAC |
PCR Primers
| Primer | Sequence |
| SU | TCTCTGTGGAGACCACATGC |
| SD | TGTATATGTGCATATTCAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
