| Gene | Nanos3 |
| Chromosome | 8 |
| Deletion Start | 84,176,528 |
| Deletion End | 84,173,849 |
| Deletion Size | 2,680 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,176,411 | 84,176,429 | GAGCCCAAGCCAAGTTCAG |
| TUP | 84,176,385 | 84,176,409 | AAGCCAGCAAGCCAGCAAGGAATCC |
| TUR | 84,176,333 | 84,176,352 | GGGACTCGCCATTGTGTTTG |
| TDF | 84,174,218 | 84,174,238 | GATGCCCTCACTTGCATACTG |
| TDP | 84,174,192 | 84,174,216 | CACAAGCTGAGCACTGCATTCTGGG |
| TDR | 84,174,161 | 84,174,181 | GCTGCCAGGAACTGACCTTAC |
PCR Primers
| Primer | Sequence |
| SU | CTCAAGGCCACATGATACCC |
| SD | TATCGCACCCAAGGTGGTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 97 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14722A-D9 | VGB6 | C57BL/6NTac | |
| 14722C-D8 | VGB6 | C57BL/6NTac | |
