14725   Fzd9     Status: ES cell colonies screened / QC positives

GeneFzd9
Chromosome5
Deletion Start135,251,026
Deletion End135,249,231
Deletion Size1,796
Genome Build38
MGI IDMGI:1313278
NCBI Gene ID14371
BAC ID414G5
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 5

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,250,293135,250,312TCCGCGTTGTGTTTCTTCTC
TUP135,250,265135,250,291ACGGCCTTCACCGTGTTCACCTTCCTG
TUR135,250,232135,250,250GCGCTCTGGGTACTGGAAC
TDF135,249,877135,249,895AAGGTGGCTGGCGATGAAC
TDP135,249,853135,249,876TGACTGGGCTCTGCTATGTAGCCA
TDR135,249,789135,249,811CCCAGTACCAGGTAGCAAGATAG

PCR Primers

PrimerSequence
SUAAAGCTGACCCACAACGTTG
SDAGAACACAGCAGTGCCACAC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR81 bp++-
TDFTDR107 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
14725A-A3VGB6C57BL/6NTac
14725A-E8VGB6C57BL/6NTac
14725A-G4VGB6C57BL/6NTac
14725A-G12VGB6C57BL/6NTac
14725A-G10VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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