| Gene | Retnlg |
| Chromosome | 16 |
| Deletion Start | 48,872,865 |
| Deletion End | 48,874,406 |
| Deletion Size | 1,542 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 16
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,873,078 | 48,873,097 | CCCTGTACCCTGGTCTAAGC |
| TUP | 48,873,101 | 48,873,124 | TAGTCCTCTCCATCCCTGCCTTCC |
| TUR | 48,873,126 | 48,873,144 | TGCTGAAGAAGCCCTGGTC |
| TDF | 48,874,242 | 48,874,262 | TGGCTATGGCTGTGGATCTTG |
| TDP | 48,874,267 | 48,874,294 | ATCCGGGATGGAAATACTTGCCACTGTC |
| TDR | 48,874,299 | 48,874,319 | GTGGCCCAGTCCATTGTTGAG |
PCR Primers
| Primer | Sequence |
| SU | AGTTCGGCTGGTTAGAGTGG |
| SD | ACGGCTTGGAGTTCACTGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 78 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14728A-E5 | VGB6 | C57BL/6NTac | |
| 14728B-F3 | VGB6 | C57BL/6NTac | |
