| Gene | 6430527G18Rik |
| Chromosome | 12 |
| Deletion Start | 86,883,894 |
| Deletion End | 86,881,557 |
| Deletion Size | 2,338 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 86,883,841 | 86,883,863 | AGTCTTGCTACCTGTGCGACCTG |
| TUP | 86,883,818 | 86,883,838 | TCGCATGCCCTGGGCCATGAT |
| TUR | 86,883,779 | 86,883,798 | TTGACGCAACCACGGCATAC |
| TDF | 86,882,063 | 86,882,082 | CTCATGTCGGTAGCAGACAC |
| TDP | 86,882,033 | 86,882,056 | CACAGCTCATTCGCCCAAGGACGG |
| TDR | 86,881,991 | 86,882,010 | CTGTTTCGCCTAGCAGATGC |
PCR Primers
| Primer | Sequence |
| SU | AGACAGCCGCAGTAGGAGG |
| SD | ATACACACCAGCAGCAGTCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 85 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14733A-C3 | VGB6 | C57BL/6NTac | |
| 14733A-F6 | VGB6 | C57BL/6NTac | |
| 14733A-G4 | VGB6 | C57BL/6NTac | |
| 14733A-G9 | VGB6 | C57BL/6NTac | |
| 14733A-H5 | VGB6 | C57BL/6NTac | |
| 14733A-H9 | VGB6 | C57BL/6NTac | |
