| Gene | Lepre1 |
| Chromosome | 4 |
| Deletion Start | 119,232,960 |
| Deletion End | 119,248,254 |
| Deletion Size | 15,295 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 119,233,532 | 119,233,550 | AGCTCTTGGCGGACTTTGG |
| TUP | 119,233,552 | 119,233,575 | AGCTTCACCCGGTTCTTATGGGCA |
| TUR | 119,233,601 | 119,233,618 | GGCTTGGGCTGTCACTAC |
| TDF | 119,248,021 | 119,248,040 | GGTCGCTGCACAAGGATGAG |
| TDP | 119,248,043 | 119,248,070 | ATGACAACAGCTAGGTCACAGTTGGGTG |
| TDR | 119,248,099 | 119,248,117 | CGGGAAGTCCAGGCTGAAG |
PCR Primers
| Primer | Sequence |
| SU | CTCGTTCTCAGTCCATTGAG |
| SD | ATCCTGGGTGCTGTATCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14734A-A10 | VGB6 | C57BL/6NTac | |
| 14734A-G6 | VGB6 | C57BL/6NTac | |
