| Gene | Pop7 |
| Chromosome | 5 |
| Deletion Start | 137,502,066 |
| Deletion End | 137,501,623 |
| Deletion Size | 444 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,502,022 | 137,502,040 | CATCGAGGCTGAGCTGGAC |
| TUP | 137,501,996 | 137,502,019 | TGTGGAGTACACCCTTCGGAAGCG |
| TUR | 137,501,925 | 137,501,947 | GCTGGGCCTTAAAGTCAGTCTTC |
| TDF | 137,501,689 | 137,501,707 | GACGAGGGTCAGGAACAAC |
| TDP | 137,501,664 | 137,501,688 | TCAGCCATCCACATCCGAGTCTTCA |
| TDR | 137,501,631 | 137,501,650 | GTCAGCCTCCCTTCAGTTAC |
PCR Primers
| Primer | Sequence |
| SU | GTTCCTCTTCCGATCCCTTC |
| SD | TGATTCCCGTCATACTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 116 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14743A-D7 | VGB6 | C57BL/6NTac | |
| 14743B-C5 | VGB6 | C57BL/6NTac | |
| 14743C-H5 | VGB6 | C57BL/6NTac | |
