| Gene | Capn10 |
| Chromosome | 1 |
| Deletion Start | 92,934,877 |
| Deletion End | 92,947,626 |
| Deletion Size | 12,750 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 92,935,275 | 92,935,295 | AGCACCATCCCACCACCTAAG |
| TUP | 92,935,297 | 92,935,322 | TTTGTCGGGTGTAATTCAGCGCAATC |
| TUR | 92,935,326 | 92,935,346 | AGAGCAAAGTGCAAAGCCAAG |
| TDF | 92,946,850 | 92,946,868 | GCTACGCCCAGGAAGTGAG |
| TDP | 92,946,870 | 92,946,892 | CGCCTCTGCCTCCTTTCTGTGGG |
| TDR | 92,946,902 | 92,946,923 | CAGGTAGGTGGAGGGAACAATC |
PCR Primers
| Primer | Sequence |
| SU | GGCTAGCACTGCCCAGTTAG |
| SD | ACCCATCCATCCAGTTGAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14746A-B6 | VGB6 | C57BL/6NTac | |
| 14746A-B1 | VGB6 | C57BL/6NTac | |
