| Gene | Mepce |
| Chromosome | 5 |
| Deletion Start | 137,786,059 |
| Deletion End | 137,782,414 |
| Deletion Size | 3,646 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 137,785,198 | 137,785,217 | CAGAGGCCAGAACCGTGATG |
| TUP | 137,785,168 | 137,785,195 | CCTCAACCCTATGAACTCAACACAGCCA |
| TUR | 137,785,122 | 137,785,139 | TGCAGGGCAGATGGAAGG |
| TDF | 137,783,339 | 137,783,358 | TGGCCCACAGGGTAACTATG |
| TDP | 137,783,302 | 137,783,328 | AGATGAGCTGGTGGATGCCCAAAGACC |
| TDR | 137,783,276 | 137,783,294 | TGAAGCACAGCACCACATC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGTGGTAGTGGCGGAG |
| SD | GTGCAGCTAGGAACAAAGGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 96 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14747A-A12 | VGB6 | C57BL/6NTac | |
| 14747A-H6 | VGB6 | C57BL/6NTac | |
| 14747A-E8 | VGB6 | C57BL/6NTac | |
| 14747A-E9 | VGB6 | C57BL/6NTac | |
| 14747A-G9 | VGB6 | C57BL/6NTac | |
