| Gene | Gan |
| Chromosome | 8 |
| Deletion Start | 117,158,138 |
| Deletion End | 117,187,491 |
| Deletion Size | 29,354 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,158,807 | 117,158,829 | CCAGCGATTGTAACCACCAAGAG |
| TUP | 117,158,843 | 117,158,866 | TTAAGCGACGCAAACCTTAGCCCA |
| TUR | 117,158,915 | 117,158,938 | CAGGAGCCCATCTCACAAATTCTG |
| TDF | 117,187,278 | 117,187,298 | GGACTTTGCGCTGCACTACTG |
| TDP | 117,187,303 | 117,187,325 | CACCACGTGCATTACCTGGCCAC |
| TDR | 117,187,336 | 117,187,354 | CGTCTCGGAAGTGGGTCTC |
PCR Primers
| Primer | Sequence |
| SU | ACACTGAGAGCACTCGGTTC |
| SD | ATCTACAGCTACAGGCTCCT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 132 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14750A-F2 | VGB6 | C57BL/6NTac | |
| 14750A-G9 | VGB6 | C57BL/6NTac | |
| 14750A-H3 | VGB6 | C57BL/6NTac | |
| 14750A-H6 | VGB6 | C57BL/6NTac | |
| 14750A-G12 | VGB6 | C57BL/6NTac | |
