| Gene | Pax7 |
| Chromosome | 4 |
| Deletion Start | 139,832,908 |
| Deletion End | 139,828,580 |
| Deletion Size | 4,329 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 139,832,018 | 139,832,040 | TCACCGAAGGGTCTTCTAGTTTC |
| TUP | 139,831,996 | 139,832,016 | TCGGCTCAGACAGCCCATGGT |
| TUR | 139,831,965 | 139,831,983 | TTGGCACGCCGTTTCTAAC |
| TDF | 139,828,866 | 139,828,886 | CGCAGGCTTACCTGGACATAC |
| TDP | 139,828,841 | 139,828,864 | TGCTCCAGGAGTGAGCCAGGTTTC |
| TDR | 139,828,810 | 139,828,829 | GCCCTTCAGGCTTGCATTTC |
PCR Primers
| Primer | Sequence |
| SU | GACCCAAAGAAAGCCACTCC |
| SD | GACAGCTGAGGCACAGGATT |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 14754A-D6 | VGB6 | C57BL/6NTac | |
| 14754A-G8 | VGB6 | C57BL/6NTac | |
| 14754A-H6 | VGB6 | C57BL/6NTac | |
